NMR Analysis of Synthetic Peptides Agonist to PTPRJ, a Receptor-Type Protein Tyrosine Phosphatase with Tumor Suppressor Activity

摘要

The Protein Tyrosine Phosphatase Receptor J (PTPRJ) is negatively involved in cellular proliferation. It represents a new interesting target for anticancer drugs [1]. Recently five PTPRJ cyclic 9-mer peptide ligands have been identified by means of phage display library screening. After synthesis and purification, all compounds have been tested in vitro. In particular, two peptides reported an interesting anti-proliferative activity after interaction to the target. The conformational properties of all compounds have been evaluated by means of NMR studies. NMR data suggested a 2:1 stoichiometry target recognition for those peptides showing antiproliferative activity.