A Novel Preparation Method For PLGA Microspheres Using Non Toxic Solvent Emulsified In Supercritical CO_2

摘要

Purpose. To generate biodegradable poly(lactic-co-glycolic acid) (PLGA) microparticles without using volatile organic solvent for a sustained protein release. Methods. The formulation of microparticles is based on the formation of an emulsion of polymer solution in CO_2 medium. Polymer solution was prepared in injectable non-volatile solvent such as glycofurol or isosorbide dimethyl ether. Moreover, encapsulation experiments were carried out using lysozyme as model protein. An experimental design was built-up to go further in the understanding of the system and to better predict the encapsulation yield. Results. Spherical microparticles were successfully generated. Encapsulation yield of lysozyme can reach up to about 85% within our chosen ranges of parameters. Conclusion. This method with the use of non-toxic solvents is suitable for the preparation of PLGA microspheres. Further characterization step will be performed to confirm its utility in controlled release of therapeutic protein.