MODULATION OF ACTIVITY OF NKR-P1A AND NKR-P1B RECEPTORS ON NK CELLS BY COMBLIKE GLYCODENDRIMERS

摘要

NKR-P1 receptor in rodents plays an important role in the potency of natural killer cells to kill tumour, or infected, or stressed cells. Several isoforms of NKR-P1 have been identified: NKR-P1A (activation receptor), and NKR-P1B, NKR-P1D (inhibitory receptors). Natural ligands for these receptors are complicated oligosaccharide structures. Simple oligosaccharides and some N-acetylated hexosamines have been recently identified as substitutes of these complex structures. When expressed on dendrimeric structures, β-D-GlcNAc or α-D-Man monosaccharides were shown to significantly discriminate between NKR-P1A and NKR-P1B receptors, the behaviour not observed for simple monosaccharides. We prepared comblike glycodendrimers bearing Tn antigen (α-D-GalNAc) and evaluated their ability to modulated activity of these receptors.