INTEGRATION OF A DROPLET MICROFLUIDIC CHIP WITH MASS SPECTROMETRY - A STEP TOWARDS HIGH THROUGHPUT CELLULAR ANALYSIS

摘要

A major bottleneck to directed evolution workflows is the throughput required to screen a large number of genomic variants for the most efficient biocatalysts [1]. Current screening methods often rely upon wellplate fluorescent readouts which become problematic when the product of interest is not inherently fluorescent; hence, 'label-free' detection is highly desirable.