BRIDGING THE GAP BETWEEN IMAGES AND MOLECULES: PARALLEL ACQUISITION OF FTMS MASS SPECTROMETRY IMAGING AND MS/MS DATA COUPLED WITH AUTOMATED AND HIGH CONFIDENCE LIPID IDENTIFICATIONS

摘要

Mass spectrometry imaging (MSI) enables visualizations of lipid distributions throughout tissues. Yet, it has struggled to translate images to underlying lipid biochemistry. A significant contributor is the lack of automated and broad MS/MS acquisition and structural identifications tool. We address this limitation via an innovative MSI approach enabling parallel acquisition of MS/MS and FTMS-MSI of every ion without prolonging MSI acquisition time.