Tandem Mass Spectrometry (MS/MS) and Fourier Transform Mass Spectrometry (FTMS) for Analysis of Substituted Polycyclic Aromatic Compounds

摘要

The purpose of the project is to develop tandem mass spectrometric techniques for the analysis of the polar fractions from the soluble organic fractions of NBS SRM 1650 diesel exhaust particulates. These fractions contain approximately 65 percent of the total mutagenic activity of the diesel particulates. The techniques developed in the project will also be applicable to the analysis of polar fractions of other environmental particulates. The instrumentation utilized is a Kratos MS-50TA which consists of a high resolution MS-I, a standard Kratos MS-50, and a low resolution MS-II, an electrostatic analyzer. Two modes of tandem mass spectrometic operation were explored during the first year of the project. The first mode is MS/MS in which ions mass selected by MS-I are allowed to fragment, or in which mass selected ions are activated for fragmentation by collision with a neutral gas, between MS-I and MS-II and the resultant fragment ions analyzed by MS-II. The second mode of operation is constant neutral loss. In this mode, MS-I and MS-II are coupled such that MS-II is set to pass an ion arising via loss of a specific mass from the ion passed by MS-I.