ANALYSIS OF MOUSE BRAIN N-GLYCANS BY (PGC-)LC-ESI-MS USING ISOTOPICALLY LABELED STANDARDS

摘要

N-glycosylation is the most complex and heterogeneous post translational modification. It is important for protein folding, stability and biological interactions of many proteins. The large number of possible isomers yielding from one glycan composition necessitates powerful separation techniques (porous graphitic carbon LC) and the use of defined standards combined with MS/MS.