Simultaneous UPLC–MS/MS analysis of two stable isotope labeled versions of sucrose in mouse plasma and brain samples as markers of blood-brain barrier permeability and brain vascular space

摘要

Highlights?Blood-brain barrier permeability is frequently measured in studies of brain disease.?Correction for brain intravascular content of permeability markers is crucial.?We propose to use two 13C-sucrose variants as permeability and vascular markers.?A UPLC–MS/MS method for analysis of the two analytes is developed.?We show that application of the vascular marker is equivalent to vascular washout.AbstractBlood Brain Barrier (BBB) permeability is frequently compromised in the course of diseases affecting the central nervous system (CNS). Sucrose is a low molecular weight, hydrophilic marker with slow permeability at the naive BBB and therefore one of the widely used indicators of barrier integrity. Our laboratory recently developed a highly sensitive UPLC–MS/MS method for stable isotope la