NATIVE MASS SPECTROMETRY TO IDENTIFY STOICHIOMETRIES AND STABILITY OF CRISPRCASCADE COMPLEXES

摘要

The CRISPR-Cas system of bacteria or archaea is an adaptive immune system against foreign DNA. The type I system uses a large ribonucleoprotein surveillance complex called 'Cascade' in which the crRNA targets the foreign DNA by base-pairing and thus triggers DNA degradation. We used native mass spectrometry (MS) to determine the protein stoichiometries of CRISPR-Cascade complexes containing longer or shorter crRNAs than the wild-type complex.