CHARACTERIZATION OF NIST MONOCLONAL ANTIBODY ON INTACT, SUBUNIT, AND PEPTIDE LEVEL WITH MONITORING OF CQA'S ON THE 6600

摘要

Monoclonal antibodies (mAbs) can be incredibly complicated with hundreds of resides, varying degrees of glycosylation and PTMs which may be critical to quality. MS can uncover a wealth of information of these mAbs with a synergistic workflow used to maximise data quality and fidelity. Here BioPharmaView and the 6600 characterize the NIST mAb on an intact, subunit, and peptide level. Additionally the MAM workflow in BioPharmaView has been used to control CQAs.