VERIFIED HILIC LC-MS/MS ASSAY FOR HIGH-THROUGHPUT TARGETED LIPDOMICS ANALYSIS

摘要

There are about 150,000 different lipid molecular species present across the biological spectrum. 'Shotgun' lipidomics is an established non-targeted approach for broad-based lipidomic analysis but it can suffer from inherent ion suppression and ambiguous identification. Herein we introduce a targeted lipidomics workflow with the combination of HILIC LC separation and multiple reaction monitoring (MRM) mass acquisition. HILIC LC chemistry provides reproducible isomer separation based on lipid classes. The Scheduled MRM type data acquisition provides high sensitivity and wide coverage (over 1200 lipid species) for lipids screening and relative quantitation with 24 min run time. The method was extensively verified and is a robust solution for a targeted lipidomics workflow.