TIMSTOF PRO AND PASEF MULTIPLYING SEQUENCING SPEED AND SENSITIVITY IN MASS SPECTROMETRY-BASED PROTEOMICS

摘要

Applications of proteomics technologies to biomedical research require further developments of mass spectrometry (MS) technology to overcome longstanding limitations in speed, sensitivity, and robustness. Trapped ion mobility spectrometry (TIMS) coupled to a quadrupole time-of-flight (qTOF) analyzer in combination with the 'Parallel Accumulation - SErial Fragmentation' (PASEF) scan mode has shown much promise in this regard. [1]