Determination of protein adducts originating from 1-methoxy-3-indolylmethyl glucosinolate using isotope-dilution UPLC-ESI-MS/MS

摘要

1-Methoxy-3-indolylmethyl (1-MIM) glucosinolate (GLS) occurring in cabbage, broccoli, and other cruciferous plants is a potent mutagen requiring metabolic activation by myrosinase present in plant cells and intestinal bacteria (1). Humans are exposed to high amounts of 1-MIM glucosinolate by consumption of Brassica vegetables. The nutrition-related exposure of humans to 1-MIMglucosinolate is usually muchhigher compared to that of known foodborne genotoxicants such as benzo[a]pyrene and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP).