Cocktail approach for microsomal CYP450 phenotyping using UHPLC-QTOF

摘要

Dioxygenation is an important biochemical reaction that often initiates the mineralization of recalcitrant aromatic contaminants such as nitroaromatic explosives, chlorinated solvents, and polycyclic aromatic hydrocarbons in the environment. To date, the extent of contaminant dioxygenation in contaminated soils and aquatic systems, is assessed from changes in pollutant stable isotope ratios using compound-specific isotope analysis (CSIA). Because such dioxygenations are often accompanied by additional oxidative side-reactions, isotopic analysis of the contaminants impedes the derivation of pathway-specific kinetic isotope effects (KIEs). The evaluation of the more indicative isotope ratios of the hydroxylated product, however, is not feasible using the popular analytical approaches with gas chromatography coupled to isotope ratio mass spectrometry.