Development of an LC-HRMS-based metabolomic approach to study methicillin-resistant Staphylococcus aureus

摘要

Antibiotic resistance often appears shortly after the introduction of a new drug as a direct consequence of its intensive use. Moreover, we are now running out of treatment options for many infections. Sometimes, confident identification of resistant strains can be difficult using conventional diagnostic techniques due to the lack of robust biomarkers of antibiotic resistance. Methicillin-resistant Staphylococcus aureus represents a major cause of hospital- and community-acquired bacterial infections. In this context, we have evaluated the usefulness of metabolomics as an approach to differentiate methicillin-resistant from susceptible S. aureus strains (MRSA and MSSA, respectively), and also to potentially highlight markers of antibiotic resistance for further detection purposes.