High-resolution peptidomic and transcriptomic profiling of spider venom: a rapid and comprehensive method for assessment of complex venom composition.

摘要

Spider venoms are complex fluids, constituted of hundreds of different components, mainly peptides and proteins but also small molecules such as acylpolyamines. Nowadays 44540 spider species, distributed in 112 families are described (Platnick, The World Spider Catalog, Version 14.5). Among these only a small number of species have been studied so far in term of venom components and proteome investigation. A major limitation in the study of spider venom is accessing sufficient amount of raw material to conduct an in-depth proteomic analysis using traditional methodology.