Variability of glucosinolates in Brassica germplasm collections

摘要

Glucosinolates are the secondary metabolites produced by the crucifer plants. In order to search for a new source of low glucosinolate more than 1200 Indian and exotic accessions of Brassica and its wide relatives obtained from National Bureau of Plant Genetic Resources, India were analyzed by HPLC comprising of 651 B. juncea, 461B. campestris/B. rapa, 35 B. napus, 10 B. nigra, 18 B. carinata, 6 B. chinensis, 63 Eruca sativa, 5 Sinapis alba, 3 Raphanus sp., 5 B. tourniforti, and 1 Crambe asyssinica,B. juncea accessions from sub-species ragosa, characteristically yielded high allyl glucosinolate as compared to Indian B. juncea accessions, which registered medium allyl and high butenyl glucosinolate. Exotic B. napus recorded low hydroxy-butenyl glucosinolate except for some. Among Indian B. napus GSL-1-SEL recorded medium glucosinolate while others had high hydroxy-butenyl. The exotic accessions of B. nigra showed high allyl glucosinolate while Indian accessions recorded medium allyl and high butenyl, although a few accessions showed hydroxyl-butenyl in place of allyl glucosinolate. There were no significant differences among B. campestris sub-types toria, yellow sarson and brown sarson. They recorded predominantly high butenyl glucosinolate, with allyl and hydroxy-butenyl being either absent or present in negligible amounts. B. chinensis showed glucosinolate profile similar to that of B. campestris/B. rapa. Among the widely related species, B. tournefortii accessions ranged from high allyl to medium butenyl to high hydroxy-butenyl, thus representing a wide variability. Raphanus species recorded high allyl, high butenyl and low hydroxyl-butenyl. B. carinata germplasms recorded high hydroxy-butenyl and very low to medium hutenyl. Eruca sativa accessions recorded varied glucosinolate profiles. All S. alba accessions were exotic collections and had butenyl as predominant glucosinolate with low to medium amount of allyl. A total of twenty six exotic accessions; twenty B.napus, two B. juncea, one B. carinata, one Crambe asyssinica, and two E.sativa have been identified having ≤30 μmoles glucosinolate. This variability in glucosinolates can be further utilized for crop improvement and to study the inheritance pattern of various glucosinolates.