Analysis of chlorination, bromination, nitration, nitrosylation, and oxidation in hemoglobin of diabetes mellitus patients by nanoflow LC-NSI/MS/MS

摘要

This highly sensitive and specific mass spectrometry assay measures the extent of PTMs in hemoglobin isolated from one drop of blood (～10 μL). The results show that the extents of chlorination at α-Tyr-24, nitration at α-Tyr-42, and oxidation at α-Met-32 and α-Met-72 in hemoglobin of diabetic patients are statistically higher than those in normal individuals. These PTMs might be used as potential biomarkers for diabetes mellitus.