Chiral separation and quantitation of cathinone related drugs by Gas chromatography Mass spectrometry

摘要

A sensitive and selective method for detection and quantitation of cathinone related compounds is developed using gas chromatography mass spectrometry (GC-MS) technique. Chiral derivatization agent, trifluoroacetyl-l-prolyl chloride (TPC) was used to achieve enantiomeric separation of cathinone derivatives. More than 30 compounds of synthetic cathinones were separated to their optical enantiomers successfully by using a 60m HP5-MS capillary column. Helium was used as carrier gas with a constant flow of 1.2 ml/min. Nikethamide which has similar chemical structure to cathinones was used as an internal standard for quantitation purposes. Twelve of the cathinones derivatives were separated in one chromatogram after they were spiked in one urine sample. Calibration curves of the selected 12 cathinones derivatives in urine and plasma were constructed in the concentration range of 5, 10, 20, 50, 100, 200 ppm. Sensitivity, selectivity accuracy, precision reproducibility and recovery of the method have been evaluated.