We confidently identified 3840 unique lysine acetylation sites across 978 acetylated wild-type E. coli proteins during growth in M9 media + sugar. The acetylation status of 274 lysines on 157 proteins showed statistically significant, glucose- and/or xylose-dependent increases (>2 fold), with median changes of 3-4 fold in the acetylome. Higher sugar supplementation (4 vs 0.4%) increased relative acetylation levels independent of the sugar source (either Glc or Xyl). Overall acetylation is not carbon source-specific but rather sensitive to carbon flux. This hypothesis could be explained as substrates acCoA and acP are common end-products of central metabolism, regardless of the carbon source. Serial Enrichment protocols for multiple acylation modifications. New SWATH-MS2-based Stoichiometry workflow for acetylation and succinylation occupancy of proteins (higher accuracy).
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机译：在M9培养基+糖的生长期间，我们自信地识别在978乙酰化野生型大肠杆菌蛋白上的3840个独特的赖氨酸乙酰化位点。在157个蛋白质上的274个赖氨酸的乙酰化状态显示出统计学上显着，葡萄糖和/或木糖依赖性（> 2倍），中值变化在乙酰胺中倍数为3-4倍。糖补充更高的糖补充（4 vs 0.4％）增加相对乙酰化水平，无关（Glc或Xyl）。总体乙酰化不是碳源特异性但对碳通量敏感。该假设可以解释为底物AccoA和ACP是中央代谢的常见终产物，无论碳源如何。用于多个酰化修饰的连续富集方案。基于新的SWATH-MS2的化学计量工作流程，用于乙酰化和琥珀酰化占蛋白质（更高的精度）。
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