Quantitative Proteomics Of Mouse Skeletal Muscles After 30 Days Of Microgravity On The BION-M1 Biosatellite

摘要

Using a robust quantitative proteomics workflow, we found SOL to be the most responsive muscle followed by EDL and VL. Moreover, SOL was the only muscle changing its fiber type composition based on relative abundance of myosin heavy chain isoforms, exhibiting a clear trend towards fast glycolytic fibers, which was confirmed by an upregulation of glycolytic enzymes and a decrease in proteins involved in lipid mobilization and fatty-acid β-oxidation. Moreover, significant proteins were also involved in protein balance, overall cellular metabolism and muscle function, as well as tissue remodelling and telomere maintenance. After a recovery phase of one week, most of microgravity-induced proteome changes were reverted. Interestingly, among the processes still affected after a recovery-phase of 8 days was glycogen biosynthesis indicating a delayed restoration of muscle glycogen (data not shown). Because inactivity-related metabolic diseases like diabetes or metabolic syndrome are commonly observed in (inactive) humans and pose a big challenge to modern-world medical care, completion of data mining with regard to overall metabolic status of the muscles will no doubt help in deciphering mechanisms underlying muscle wasting disorders, which will favour definition of possible new countermeasures.