Optimization of tissue extraction for GCMS metabolomic analysis reveals altered metabolism of barbiturates in a polycystic kidney disease model

摘要

Since for both kidney and liver tissues, the 5 mg extract had a significantly greater number of compounds and peak area of the TIC than 2 mg, and no significant increase in compounds or TIC was seen for the 10 mg extractions, 5 mg of tissue were used for kidney and liver extractions for future metabolomic analyses of organ tissue. In this optimization, it was also found that Lewis polycystic kidney rats appear to metabolize barbiturate compounds differently to age- and sex-matched controls, which may alter broader metabolite profiles. To avoid interpretation of the euthanasia method rather than the disease biochemistry of the polycystic kidney rat, future studies adopted an exsanguination euthanasia method. The benefit of an exsanguination method is two-fold: 1) no euthanasia drug is introduced and; 2) a near total blood volume is removed from the animal allowing organs free from circulating blood to be harvested.