Yeast proteome dynamics are revealed by hyperLOPIT: a high resolution spatial proteomics approach

摘要

We have used dynamic hyperLOPIT to map steady-state protein localisation to multiple subcellular organelles in yeast in two separate culture conditions. Our data indicates a small overlap with published data and reveals interesting insight as to the cellular response of the spatial proteome to nitrogen starvation. Future work includes application of hyperLOPIT to the temporal response of the yeast spatial proteome to starvation.