Characterization of Histone H4 Post-translational Modification in the Microalgae Chlamydomonas reinhardtiiby Top Down Mass Spectrometry

摘要

Identification of histone H4, with the most abundant mass presentas +72 Da above the theoretical mass of the unmodified protein, which includes the presence of a acetylation (localized N-terminally), a monomethylation (localized to lysine 79), and an oxidation (localized to methionine 84). Conclusive identification of a monomethylation on lysine 79 localized by the presence of the y22~(2+) ion(Δm = 0 Da) and the y24~(2+) ion (Δm = +14 Da) (Figure 2). The different degrees of methionine 84 oxidation with in the cell cycle specific to the histone H4 protein, implying in vivo oxidation.