Mapping proteolytic peptide production rates in plasma using stable isotope labeled proteins from the SILAC-labeled HepG2 secretome

摘要

Demonstrated the potential for using HepG2 cells for generation of stable isotope labeled plasma proteins for MS-based quantification. ＞60 intact stable isotope labeled plasma proteins generated, including complex apolipoproteins from non-depleted plasma. Strategies to improve coverage of lower abundant proteins are being explored. SILAC HepG2 peptide cocktail allowed ＞3500 peptides from ＞60 plasma proteins to be monitored over time with 745 suitable PABST H/L peptide pairs identified. Broad range of isotopically labeled peptides formed (596), with high PABST suitability scores for PRM based experiments.