Evaluation of 25-hydroxy Vitamin D Extraction using Phospholipid Depletion Plate Technology and Method Comparison using Automated Sample Preparation

摘要

This poster demonstrates the applicability of ISOLUTE PLD+ for the extraction of 25-hydroxy vitamin D from 100 or 150 μL of serum. Good extraction efficiency, protein and phospholipid removal was afforded by the technique. Excellent linearity, and r2 values from PBS/BSA and Chromsystems calibrated serum along with good correlation data to DEQAS reported patient samples. Successful method implementation was achieved on to the Extrahera automated sample preparation platform.