A top-down mass spectrometry assay for effective protein denaturation based upon dimethylation of primary amines

机译：基于原发性胺的二甲基化的预上质谱法测定有效蛋白质变性

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摘要

Reductive alkylation reactions on intact proteins introduce heterogeneity. Further improvement in protocols wiill be necessary for assessment of denaturation.

机译：完整蛋白质上的还原烷基化反应引入异质性。协议Wiill的进一步改善是对变性的评估所必需的。

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《ASMS Conference on Mass Spectrometry and Allied Topics》|2015年||共1页
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Joseph Capri; Whitaker Cohn; Sara Bassilian; Kym F. Faull; Julian Whitelegge;
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中图分类 TB994.5-53;
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1. Top-down analysis of small plasma proteins using an LTQ-Orbitrap. Potential for mass spectrometry-based clinical assays for transthyretin and hemoglobin [J] . Théberge R., Infusini G., Tong W., International journal of mass spectrometry . 2011,第2a3期

机译：使用LTQ-Orbitrap对小血浆蛋白进行自上而下的分析。基于质谱的运甲状腺素蛋白和血红蛋白临床检测的潜力
2. A mesofluidic platform integrating on-chip probe ultrasonication for multiple sample pretreatment involving denaturation, reduction, and digestion in protein identification assays by mass spectrometry? [J] . J. D. Nunes-Miranda, Cristina Nú?ez, Hugo M. Santos, The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry . 2014,第5期

机译：集成了片上探针超声处理的中流平台，用于通过质谱分析蛋白质鉴定测定中的变性，还原和消化的多样品预处理？
3. Intact protein profiling in breast cancer biomarker discovery: Protein identification issue and the solutions based on 3D protein separation, bottom-up and top-down mass spectrometry [J] . BouchalP., DvorakovaM., ScherlA., Proteomics . 2013,第7期

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4. A top-down mass spectrometry assay for effective protein denaturation based upon dimethylation of primary amines [C] . Joseph Capri, Whitaker Cohn, Sara Bassilian, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

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5. Mass spectrometry-based structural proteomics: Bottom-up protein footprinting and top-down native electrospray of protein assemblies. [D] . Zhang, Hao. 2011

机译：基于质谱的结构蛋白质组学：自下而上的蛋白质足迹和自上而下的蛋白质组装的自然电喷雾。
6. Top-Down Analysis of Small Plasma Proteins Using an LTQ-Orbitrap. Potential for Mass Spectrometry-Based Clinical Assays for Transthyretin and Hemoglobin [O] . Roger Théberge, Giuseppe Infusini, Weiwei Tong, -1

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7. Top-down analysis of small plasma proteins using an LTQ-Orbitrap. Potential for mass spectrometry-based clinical assays for transthyretin and hemoglobin [O] . Roger Théberge, Giuseppe Infusini, Weiwei Tong, 2011

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A top-down mass spectrometry assay for effective protein denaturation based upon dimethylation of primary amines

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