Metaproteomic analysis of human cervical-vaginal fluid in residual Pap tests: Insights into the cervical microbiome

摘要

We have successfully characterized the profile of microbial flora in phylum, family, genus and species level in Pap test of women with normal cytology. Using MSI tools, we have developed a novel FASTA protein database consisting of translated reference genomes from 130 microbial organisms from Human Microbiome Project (HMP) concatenated with the human proteome database (UniProt isoform canonical database) and 3-frame cDNA protein sequences in human urogenital tract. Using a two step search method4 for data analysis, avoids false positive matches, resulted in identification of higher number of high confidence peptides.