Application of Capillary Electrophoresis coupled to Quadrupole Time-of-Flight Mass Spectrometry for the Analysis of Immunoconjugates

摘要

In this study, a CE-Q-TOF MS method for the characterization of scFv conjugates labeled with two selected small reporter molecules was developed. Under the CE conditions employed, separation between the various degrees of conjugation of the two immunoconjugates applied was obtained. The accurate mass measurement combined with MassHunter BioConfirm Software enabled assignment of the specific conjugates. The results suggest that CE/MS is a potential tool for the characterization of immunoconjugates.