Characterization of Clinically-Relevant Stable Isotope Labeled Recombinant Proteins For Use As Internal Standards in Quantitative MS Workflows

摘要

We have characterized stable isotope labeled (SIL) full length versions of IGF1, Thyroglobulin, and APOA1 for utility as internal standards in quantititative MS workflows. These clinically-relevant SIL proteins can be spiked at the initial stage of the analytical workflow to significantly minimize process variations and therefore represent a more suitable choice for use as an internal standard in quantitative MS-based protein assays.