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Analyzing a complex sample by MS/MS using isotopically-labeled standards
Analyzing a complex sample by MS/MS using isotopically-labeled standards
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机译:使用同位素标记的标准品通过MS / MS分析复杂样品
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摘要
A method and corresponding apparatus are disclosed for analysis of a peptide-containing sample. The sample is prepared by adding isotopically-labeled peptides corresponding to endogenous peptides of interest, and the prepared sample is analyzed by liquid chromatography-mass spectrometry (LCMS). Detection in a high-resolution, accurate mass (HRAM) MS1 spectrum of a precursor ion matching an isotopically-labeled peptide triggers acquisition of an MS/MS spectrum (preferably acquired in an ion trap or other fast mass analyzer) to determine if a product ion is present matching a characteristic product ion (e.g., the y1 ion) of the isotopically-labeled peptide. If the characteristic product ion is present, then a HRAM MS/MS spectrum is acquired for detection and quantitation of the corresponding endogenous peptide.
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机译:公开了一种用于分析含肽样品的方法和相应的设备。通过添加与目标内源肽相对应的同位素标记的肽来制备样品,并通过液相色谱-质谱(LCMS)分析制备的样品。在高分辨率,精确质量(HRAM)MS1光谱中检测与同位素标记的肽相匹配的前体离子会触发MS / MS光谱的采集(最好在离子阱或其他快速质量分析仪中采集)以确定产品是否存在与同位素标记的肽的特征产物离子(例如y 1 Sub>离子)匹配的离子。如果存在特征产物离子,则获取HRAM MS / MS谱图以检测和定量相应的内源肽。
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