A data-independent acquisition strategy on the Q Exactive for monitoring GALNT2-mediated APOCIII glycosylation in cell culture

摘要

MS analysis confirmed APOCIII Thr94 is the major glycosylation site for GALNT2. MS1-based quantification of glycosylation events is in agreement with the pyrophosphate-based method. The in vitro assay can screen for inhibitors of GALNT2 activity.