Targeted and Untargeted Mass Spectrometry for Identification of Metabolomic Changes in a Human Epigenetic Model of Chronic Stress

摘要

Both targeted and untargeted metabolomics approaches found that PCs differed between the 'high' and 'low' methylation groups. The tentative identification of PCs, PEs, Pis, and PS that are altered in the high methylation group may suggest a relationship of HPA dysregulation to phospholipid biosynthesis (and this has been previously suggested in the literature). Pathway mapping and follow-on targeted experiments may help determine whether the observed differences correspond to a systematic alteration in metabolism. Additional models of chronic stress, HPA hyperactivity, and GR promoter methylation will be tested to determine the validity and applicability of the findings. These include transgenerational murine models and large cohorts of human patients with burnout or exposure to very high stress/trauma.