Identification of Unknown Metabolites in Chlamydomonas reinhardtii with Accurate Mass GC-QTOF Mass Spectrometry

摘要

The published MS-based unknown analysis workflow was successfully applied to identify Chlamydomonas reinhardtii unknown metabolites. 1. Unknowns #3 and 4 were not identified due to lack of candidates and commercially available standards. The workflow was improved to permit confident elucidation of the molecular ion, further limit the number of candidate elemental formulas, and appropriately reflect derivatized structure.