A TOOL FOR QUANTIFYING AND VISUALIZING HIGH-RESOLUTION MS/MS AND MS SPECTRA FROM DATA-INDEPENDENT ACQUISITION EXPERIMENTS

摘要

Recently, we have proposed a new method for systematically monitoring changes to the various proteoforms using a targeted approach where predefined m/z "strips" corresponding to specific combinations of peptide, PTM equivalents and charge states are sequentially isolated, fragmented with ETD, and analyzed in the Orbitrap. This approach expands upon previous strategies for data independent acquisition such as SWATH. To take full advantage of the acquired data, we have developed a new tool to visualize and compare these datasets. This tool is universally applicable to high resolution data regardless of fragmentation techniques. It allows us to display data online and navigate the data with the same ease you can navigate Google Maps.