Non-covalent complexes have been extensively studied using ESI or MALDI mass spectrometry. DNA-peptide interaction presents a strong interest in interactomic science as these interactions are involved in gene regulation or expression. Different gas phase studies on peptide-DNA interactions have been performed, which evidenced the ion-pair interactions of R or K residues and the DNA phosphate groups (1,2,3,4). In this study, the origin of interactions existing between basic residues in peptides and DNA phosphate groups was investigated. Experimental observations gave evidences of the role played by zwitterions on complex stabilisation and the regulation under coulombic repulsion on complex production, which modified the DNA phosphate group acidity. To further investigate to DNA-peptide interaction duplex complexes with peptides are also studied.
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