Maximizing Performance of Sub-2 um Packed Nanobore LC Columns: Minimizing Extra-Column Variance

摘要

All of the post-column, high voltage connection devices tested yielded stable spray throughout the gradient. Minimizing or eliminating post-column volume is a key consideration in the nanobore LC flow path. Maintaining a post-column flow path having an ID less than or equal to the column ID is critical. A larger ID than that of the column is to be avoided. The PicoClear Conductive Union maintained optimal peak shape with a 75 (mu)m ID column packed with sub-2 (mu)m particles. The increase in peak area will be explored for quantitative peptide analysis in determining LOD, LOQ, and linearity.