The cell cycle dependent phosphoproteome and proteome analyzed by quantitative proteomics

摘要

Progression through the cell cycle is tightly regulated by phosphorylation of specific signaling molecules such as kinases and cyclins. Although cell cycle dependent phosphorylation events have been studied intensively for decades the majority of the important regulated phosphorylation sites still remain to be identified. We recently showed that SILAC based phosphoproteomics is capable of quantifying several thousand phosphorylation sites in response to cell stimulation (Olsen et al, Cell 2006). Here we combine this quantitative phosphoproteomics technology with quantitation at the proteome level. We have applied this methodology in synchronized HeLa S3 cells at six different time points and quantified several thousand proteins and phosphopeptides across the cell cycle.