Following the metabolic and morphological changes of individual Bacillus atrophaeus cells during the sporulation process using Bioaerosol Mass Spectrometry

摘要

Gram positive bacteria in the genera Bacillus and Clostridium are able to undergo physical and biochemical changes during periods of starvation and stress in order to form dormant and robust endospores for survival. Here, a simple set of experiments is presented in order to introduce and demonstrate the potential utility of Bioaerosol Mass Spectrometry (BAMS) for the study of cell development processes at the single cell level, using the model of B. atrophaeus endospore formation.