Using Fe_3O_4 Particles as the Matrix and Concentrating Probe for SALDI-MS Analysis of Peptides and Proteins

摘要

Tanaka et al. first reported cobalt nanoparticles mixed with glycerol as a two phase-matrix to assist laser desorption/ionization mass spectrometric analysis for proteins in the1980s. Sunner et al. and Dole et al. later demonstrated that micro-sized graphite powder mixed with glycerol could be used as an effective matrix. Sunner et al. termed it as "surface-assisted laser desorption/ionization mass spectrometry" (SALDI MS) to distinguish it from the use of organic chemicals as the matrix discovered by Karas and Hillenkamp. Hillenkamp and co-workers investigated a series of inorganic particles mixed with glycerol as the SALDI matrix. Herein, we demonstrated the feasibility of using silanized Fe_3O_4 particles as the SALDI matrix and affinity probe to develop an affinity based mass spectrometry.