Isolation and purification of intact Mycobacterium avium subspecies paratuberculosis from intestinal sections of cattle and goats

摘要

Mycobacterium avium subspecies paratuberculosis (MAP), the causative agent of Johne's disease in ruminants has been purified from infected cattle and goat tissue for the specific purpose of proteomic analysis. After removal of luminal contents, the submucosa and mucosa of ileal and jejunal sections were harvested and MAP extracted from macrophages by hypotonic lysis, sonication and differential centrifugation. This method is also suitable for DNA extraction from mycobacteria.