Validation of commercially available ELISA tests for the serodiagnosis of paratuberculosis in Germany

摘要

In preparation of a national paratuberculosis guideline, which was implemented recently, a validation study was performed including four commercial ELISA tests. The tests are based on different Map antigens, a protoplasmic antigen (test A), a whole cell antigen (test B), a cell wall antigen (LAM, test C) and a protoplasmic antigen of a Mycobacterium avium subspecies (test D). Two tests include an absorption step of the samples with M. phlei (test A and B) and two tests are also approved for milk samples (test A and C).