In vitro lung cell culture studies of particle-induced proinflammatory responses

摘要

Recent work with soil-derived mineral dusts and manufactured nanoparticles will be discussed to illustrate the types of toxicological information that can be obtained from treatment of cell cultures with low-solubility particles. Readily inhaled particulate matter (PM), which includes the enriched PM2.5 fraction of certain soil dusts or modified manufactured nanoparticles, was found to cause release of the proinflammatory cytokine IL-6 to induce cell death in human lung cell cultures. There is a need to include geological particulate matter when assessing adverse health effects of polluted air. Extensive prior cell culture research has been done by toxicologists to understand the effects and mechanisms of inhaled particles. Various surfacemodifying treatments, including heat and leaching, were used to identify the particle components or characteristics that induced the cell signaling response.