TIME-DEPENDENT SPECTRAL CHANGE OF CHEMILUMINESCENCE FROM FIREFLY LUCIFERIN IN DEOXYGENATED DIMETHYL SULFOXIDE

摘要

The molecular mechanism and light emitters of the chemiluminescence (CL) of firefly luciferin (Ln) have been interpreted as outlined in Fig.I.1'3 The oxygenation of Ln i° *ne Presence of a strong base (e.g., BuOK) produces a dioxetanone, which successively decomposes to form an electronically-excited oxyluciferin (Oxyln*).4'5 Oxyln* emits green- or red-colored light depending on its ionized state: a monoanion of Oxyln* in the keto form emits red-colored light, while its mono-and/or di-anion in the enolic form emits green-colored light at high base concentrations.