WNT-4 Expression Is Increased in Fibroblasts After TGF-Betal Stimulation and During Fetal and Postnatal Wound Repair

摘要

Wnt-4 is a mitogen expressed during postnatal repair and scar formation. In E17 fibroblasts after TGF-betal stimulation, Wnt-4 expression increases 4-fold at 1 hour (p<0.05) and peaks with an 11-fold increase at 2 hours (p<0.05). By 24 hours, expression decreases to 2-fold baseline levels (p<0.05). In postnatal fibroblasts, Wnt-4 expression also increases after TGF-beta stimulation, but peak expression is larger and relatively delayed with a 17-fold increase at 12 hours (p<0.005). Expression levels at 24 hours are still 4-fold greater than baseline (p<0.05). Small increases in Wnt-4 expression (<2-fold) occur during both fetal scarless and postnatal scarring mouse wound repair. Our data suggests TGF-beta directly increases Wnt-4 expression in fetal and postnatal fibroblasts, and that Wnt-4 is increased in both fetal and postnatal repair.