IMMOBILIZATION OF GLUCOSE OXIDASE ON POLYPYRROLE FILM VIA SUPERCRITICAL FLUID TECHNOLOGIES

摘要

The aim of this work is to immobilize a biological component on a conducting polymer via supercritical fluid technologies for further use in the preparation of biosensors. For this purpose, glucose oxidase (GOX) is chosen as the biological component and polypyrrole (PPY) as the immobilization matrix. The effect of supercritical carbon dioxide (scCO_2) on electrochemically synthesized PPY are monitored by environmental scanning electron microscopy (ESEM). Supercritical Fluid Impregnation is used to immobilize the GOX onto the PPY film. Four different impregnation mediums are used, mainly as dry GOX and dry PPY (Dry), dry GOX and dry PPY with water vapor (Semi-Dry), GOX and PPY in water (Wet), and GOX and PPY in buffer (Wet-b). GOX immobilized PPY films are analyzed via ESEM and enzymatic activity methods. In Dry and Semi-Dry procedures (up to 150 bar, 40°C and 72 hr), the activity of enzyme immobilized into or onto the PPY film was not observed, whereas films processed in Wet and Wet-b experiments enzymatic activity was observed. The maximum activity was observed for GOX immobilized PPY in the Wet-b medium (150 bar and 40°C). In Wet medium (150 bar and 40°C), as the impregnation time increased (5-72 hr), the amount of enzyme immobilized on the film increased hence the activity was enhanced.