Mapping Molecular Landscapes Inside Cells

摘要

Electron Tomography (ET) is uniquely suited to obtain three-dimensional (3-D) images of large pleiomorphic structures, such as supramolecular assemblies, organelles, or even whole cells. While the principles of ET have been known for decades, its usehas gathered momentum only in recent years. Technological advances (namely computer controlled transmission electron microscopes and large area CCD cameras) have made it possible to develop automated data acquisition procedures. This, in turn, allowed toreduce the total electron dose to levels low enough for studying radiation sensitive biological materials embedded in vitreous ice. As a result, we are now poised to combine the power of high-resolution 3-D imaging with the best possible preservation ofthe specimen.