MID-INFRARED SPECTROSCOPIC MONITORING OF ENZYME REACTION ASSOCIATING WITH IONIC DISSOCIATIVE METABOLITES

摘要

A simultaneous quantification method of concentrations of ionic dissociative materials and pH value in the mixture solutions was developed using the mid-infrared spectra of the ionic components extracted from the aqueous solutions. Additionally, we constructed an enzyme reactor accessory equipped with an FT-IR system for the continuous and non-destructive measurement and monitored the enzyme reaction process from glucose 6-phosphate to fructose 6-phosphate with D-glucose-6-phosphate ketol-isomerase.The developed quantification method was applied to the spectroscopic information of the reactant mixture, and the simulated time course of the reactant concentrations and pH value consistently represented the theoretical ones.