DETECTION OF MASTITIS PATHOGENS DIRECTLY FROM MILK BY REAL-TIME POLYMERASE CHAIN REACTION

摘要

Mastitis is the most common infectious disease affecting dairy cows and is the most economically important disease of the dairy industry. Identification of bacterial pathogens in milk from a cow with mastitis is regarded as the definitive diagnosis ofintramammary infections. Current methods for routine identification and confirmation of mastitis pathogens requires a minimum of 48 hours. Identification of bacteria utilizing DNA-based technology could eliminate analysis of phenotypic characteristics utilizing biochemical tests, serotyping and enzymatic profiles and provide an identification in 24 hours or less. Real-Time Polymerase Chain Reaction (PCR) is a relatively new DNA based technique that monitors amplification of target DNA in real-time by monitoring florescence. The objective of this study was to develop a Real-Time PCR procedure for detection of Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis, Streptococcus dysgalactiae subsp dysgalactiae and Listeria monocytogenes directly from milk.