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Inactivation of biofilm-forming bacteria using cold atmospheric plasmas and potential application for decontamination of fresh foods

机译:使用冷气氛等离子体的生物膜形成细菌的灭活和潜在应用净化新鲜食品

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Summary form only given. This work aims at evaluating the prospects for using cold atmospheric plasmas to decontaminate fresh produce. Nonthermal atmospheric plasmas have mainly been studied in the past as methods for killing pathogens on surfaces either in medical or environmental contexts. Most of these studies are based on cell inactivation on surfaces such as glass, plastics and metals which do not usually support cell growth. In food decontamination, there is the complication of interaction occurring between microbial cells and the surface. This interaction can take many different forms: micro-organisms may adsorb to the surfaces of food and simply remain attached to it during processing as is the case with abiotic materials. Alternatively, adherent organisms may exploit nutrients present on the surface of foods and go on to proliferate at the surface. Others may, under certain conditions, exude polysaccharides which serve to protect them from environmental stresses and, incidentally, makes it more difficult to detach them from surfaces. This three-dimensional matrix of polysaccharides and micro-organisms is referred to as a 'biofilm.' In order to facilitate rigorous evaluation of our decontamination methods, we have chosen to conduct our initial experiments using a model system. In future work once we have optimised the operation of our plasma, we intend to use naturally contaminated foods and additionally, to evaluate the effects of cold plasmas on key nutrients in the food. Our model system comprises the biofilm-forming bacterium Pantoea (Enterobacter) agglomerans growing on synthetic membranes to simulate bacterial growth on the surface of plant tissue. As the biofilm develops with time, the production of polysaccharides becomes more extensive and cells contained within the matrix are shielded to an increasing degree. We present data on the kinetics of inactivation of Pantoea agglomerans in biofilms of different ages by cold atmospheric plasmas generated in a helium-oxygen mixture and with a radio-frequency excitation. Data is also presented on the UV treatment of such biofilms in order to permit comparison of both methods of food decontamination.
机译:摘要表格仅给出。这项工作旨在评估使用冷大气等离子体来净化新鲜农产品的前景。过去主要研究了非热大气压等离子体作为在医学或环境背景下杀死表面的病原体的方法。这些研究中的大多数是基于细胞灭活,如玻璃,塑料和通常不支持细胞生长的玻璃,塑料和金属。在食品去污中,在微生物细胞和表面之间存在相互作用的并发症。这种相互作用可以采取许多不同的形式:微生物可以吸附到食物的表面上,并且在加工过程中简单地保留在其上,如非生物材料的情况。或者,粘附生物可以利用存在于食物表面上的营养物,并继续在表面上增殖。在某些情况下,其他可能在某些条件下,用于保护它们免受环境压力的散热性,并且顺便提一下,使其更加困难地脱离表面。这种多糖和微生物的三维基质被称为“生物膜”。为了促进严格评估我们的去污方法,我们选择使用模型系统进行我们的初始实验。未来工作一旦我们优化了我们的等离子体的运作,我们打算使用天然受污染的食物,另外,评估冷等离子体对食物中的关键营养素的影响。我们的模型系统包括生长在合成膜上的生物膜形成细菌(肠杆菌)聚氨酯,以模拟植物组织表面上的细菌生长。随着生物膜随时间的发展,多糖的产生变得更加广泛,并且基质内包含的细胞被屏蔽到增加程度。我们通过在氦 - 氧混合物中产生的冷大气压等离子体和射频激发,呈现对不同年龄的生物膜灭活动力学的数据。还介绍了这种生物膜的UV处理中的数据,以便允许比较两种食物去污方法。

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